The RETRO-TEK SIV p27 Antigen ELISA is for research use only and is not intended for in vitro diagnostic use. The RETRO-TEK SIV p27 Antigen ELISA is an enzyme linked immunoassay used to detect Simian Immunodeficiency Virus (SIV) p27 antigen in research specimens, including cell culture media. It can also be used to monitor the purification and biochemical behavior of SIV. Furthermore, the assay may augment or supplant reverse transcriptase measurements traditionally employed to detect the presence of SIV. Such enzymatic measurements are not SIV specific.

Microwells are coated with a monoclonal antibody specific for the p27 gag gene product of SIV. Viral antigen in the specimen is specifically captured onto the immobilized antibody during specimen incubation. The captured antigen is then reacted with a hightiter polyclonal anti-SIV antibody conjugated to biotin. Following a subsequent incubation with streptavidin peroxidase, color develops as the bound enzyme reacts with the substrate. Resultant optical density is proportional to the amount of SIV p27 antigen present in the specimen.

+2? to +8? C.
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