Glutathione reductase (GR, ECn. 1.6.4.2) is a flavoprotein that is required for the conversion of oxidized glutathione (GSSG) to reduced glutathione (GSH). At the same time, it oxidizes nicotinamide adenine dinucleotide phosphate (NADPH). This universally present enzyme is essential for the maintenance of reduced glutathione (GSH) levels in vivo(1). Reduced glutathione plays an important role in oxidoreduction processes and detoxification of H2O2 and organic peroxides, which are substances produced in large quantities during inflammatory processes in living cells(2). Glutathione reductase therefore plays a major role in glutathione peroxidase (GPx) and glutathione stransferase (GST) reactions as an adjunct in the control of peroxides and free radicals(3). When levels of catalase, another universally present antioxidant enzyme, are decreased, the glutathione dependant enzymes become activated(4). A deficiency of glutathione reductase is characterized by hemolysis owing to the increased sensitivity of erythrocyte (RBC) membranes to H2O2 that lead to osmotic fragility(5). This reaction is thus required for the stability and integrity of red cells. The Glutathione Reductase Assay Kit provides an indirect and highly reproducible method of quantifying the activity of total cellular glutathione reductase. The activity of the enzyme is an important measure of the antioxidant status of the cell. Oxidative stress has been implicated in aging and in the pathogenesis of a number of disorders. The extent of injury is generally related to an increase, or decrease of one or more free radical scavenging enzymes(2). High levels of glutathione reductase have been found in erythrocytes from patients with rheumatoid arthritis (6). The Glutathione Reductase Assay Kit is for Research Purposes Only.

Store the assay buffer at 2-4 °C. NADPH and oxidized glutathione should be stored at -70°C. The components of the Kit are stable for 1 year when stored properly.

+2° to +8° C.
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