This murine monoclonal antibody reacts with Human Immunodeficiency Virus Type 1 (HIV-1) p17 protein. The antibody was raised protein-by immunizingfree cell culture supernatant by protein A chromatography. BALB/c mice with purified HIV-1 (Strain IIIB) lysate. The antibody epitope has been m is of the IgG2a subclass and apped to amino acids 12has been purified from -19

Anti-HIV-1 p17 Clone 32/5.8.42 exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence. When tested by Western blot with viral lysates, antibodindirect immunostaining techniques to detect HIVy shows a strong reaction with -1 HIVr cultured HIV-1 p17 core protein. The antibody may be used in -1 infected cells. Studies on core antigen synthesis and metabolism can be performed by Western blot or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein. Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity. Optimal dilution of antibody must be determined experimentally by the investigator.

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